INHIBITION OF MOBILITY OF NEUTROPHILS OF SURGICAL PATIENTS WITH PURULENT WOUNDS AND WOUND INFECTION AS AN INDICATOR OF INTOXICATION OF THE ORGANISM
https://doi.org/10.25199/2408-9613-2014-1-2-38-43
Abstract
The method for automatic recording and analysis of motor activity of neutrophils based upon the Magiscan 2A image analysis system allows conducting express diagnostics of motility of neutrophils of patients. It was shown that cells with various speed of movement on glass are contained in the population of neutrophils. The average speed of movement of neutrophil population is a stable indicator of movement activity of the population and can serve as an objective parameter of the functional state of neutrophils with various diseases. Patients with purulent surgical infections manifest correlation between inhibition of neutrophil motility and deteriorating of clinical status of patients (speed in μm / min, healthy donors – 8.8; patients of average severity – 6.5; serious patients – 3.3). Blood serum of serious patients had an inhibitory effect on the migration of neutrophils from healthy donors. Increasing of the average speed of movement of neutrophils from 3.4 to 7.1 μm / min in 1 to 2 days after a radical surgery (amputation) was demonstrated among 11 patients with the diabetic gangrene of the foot. Previously, we have shown the existence of reciprocal relationships between motility of neutrophils and their activation. Inhibition of motility of neutrophils with surgical diseases can be explained by their priming or activation.
About the Authors
А. А. Galkin
Clinical and Diagnostic Department, A. V. Vishnevskiy Institute of Surgery, Ministry of Health of Russia
Russian Federation
27 Bolshaya Serpukhovskaya St., Moscow, 117 997, Russia
Russian Federation
27 Bolshaya Serpukhovskaya St., Moscow, 117 997, Russia
V. S. Demidov
Clinical and Diagnostic Department, A. V. Vishnevskiy Institute of Surgery, Ministry of Health of Russia
Russian Federation
27 Bolshaya Serpukhovskaya St., Moscow, 117 997, Russia
Russian Federation
27 Bolshaya Serpukhovskaya St., Moscow, 117 997, Russia
O. A. Zakharova
Clinical and Diagnostic Department, A. V. Vishnevskiy Institute of Surgery, Ministry of Health of Russia
Russian Federation
27 Bolshaya Serpukhovskaya St., Moscow, 117 997, Russia
Russian Federation
27 Bolshaya Serpukhovskaya St., Moscow, 117 997, Russia
References
1. Alves-Filho J.C., Spiller F., Cunha F.Q. Neutrophil paralysis in sepsis. Shock 2010;34 Suppl 1:15–21.
2. Bone R.C. Toward a theory regarding the pathogenesis of the systemic inflammatory response syndrome: what we do and do not know about cytokine regulation. Crit Care Med 1996;24:163–72.
3. Галкин А.А., Демидова В.С. Повреждение защитных функций нейтрофилов на ранней стадии ожоговой болезни. Успехи современной биологии 2012;132(3):297–311. [Galkin A.A., Demidova V.S. Damage of neutrophils protective functions at the early stage of burn disease. Uspekhi sovremennoy biologii = Progress of Modern Biology 2012;132(3):297–311 (In Russ.)].
4. Галкин А.А. Подвижность нейтрофилов в норме и патологии. Дис. ... д-ра биол. наук. М., 1997. 200 с. [Galkin A.A. Motility of neutrophils in the norm and in pathology. Thesis ... of MD. Мoscow, 1997. 200 p. (In Russ.)].
5. Fowler A.A., Fisher B.J., Centor R.M. et al. Development of the adult respiratory distress syndrome: progressive alteration of neutrophil chemotactic and secretory pro-cesses. Am J Pathol 1984;116(3):427–35.
6. Davis J.M., Meyer J.D., Barie P.S. et al. Elevated production of neutrophil leukotriene B4 precedes pulmonary failure in critically ill surgical patients. Surg Gynecol Obstet 1990;170(6):495–500. 7. Tellado J.M., Christou N.V. Critically ill anergic patients demonstrate polymorphonuclear neutrophil activation in the intravascular compartment with decreased cell delivery to inflammatory focci. J Leukoc Biol 1991;50(6):547–53.
7. Rivkind A.I., Siegel J.H., Littleton M. et al. Neutrophil oxidative burst activation and the pattern of respiratory physiologic abnormalities in the fulminant post-traumatic adult respiratory distress syndrome. Circ Shock 1991;33(1):48–62.
8. Галкин А.А., Тимин Е.Н., Карелин А.А. Методы исследования подвижности ней- трофилов (обзор литературы). Клиническая лабораторная диагностика 2003;1: 22–5. [Galkin A.A., Timin E.N., Karelin A.A. Method of studying of neutrophils motility (review of references). Klinicheskaya laboratornaya diagnostika = Clinical Laboratory Diagnostics 2003;1:22–5 (In Russ.)].
9. Туманов Е.А., Галкин А.А., Филиппов М.М. и др. Метод автоматической регистрации и анализа миграции лейкоцитов на базе системы обработки изображений. Бюллетень экспериментальной биологии и медицины 1990;6:594–7. [Tumanov E.A., Galkin A.A., Filippov M.M. et al. Method of automatica regiatration and analysis of migration of leukocytes on the basis of the image processing system. Byulleten experimentalnoy biologii i mediziny = Bulletin of Experimental Biology and Medicine 1990;6:594–7 (In Russ.)].
10. Sroka J., Kordecka A., Wlosiak P. et al. Separation methods for isolation of human polymorphonuclear leukocytes affect their motile activity. Eur J Cell Biol 2009; 88(9):531–9.
11. Галкин А.А., Туманов Е.А., Филиппов М.М. и др. Подвижность нейтрофилов у больных с раневой инфекцией. Бюллетень экспериментальной биологии и медицины 1991;111(5):491–5. [Galkin A.A., Tumanov E.A., Filippov M.M. et al. Motility of neutrophils of patients with would infections. Byulleten experimentalnoy biologii i mediziny = Bulletin of Experimental Biology and Medicine 1991;111(5):491–5 (In Russ.)].
12. Wagner J.G., Roth R.A. Neutrophil migration mechanisms, with an emphasis on the pulmonary vasculature. Pharmacol Rew 2000;52(3):349–74.
13. Барсуков А.А., Годков М.А., Земсков В.М. и др. Роль праймированных нейтрофилов в повреждении паренхиматозных органов и развитии воспалительной патологии. Успехи современной биологии 2004:124(6):542–54. [Barsukov A.A., Godkov M.A., Zemskov V.M. et al. The role of primed neutrophils in damaging of parenchymal organs and development of inflammatory pathologies. Uspekhi sovremennoy biologii = Progress of Modern Biology 2004;124(6):542–54 (In Russ.)].
14. Cowburn A.S., Condiffe A.M., Farahi N. et al. Advances in neutrophil biology. Clinical implications. Chest 2008;134:606–12.
15. Галкин А.А., Туманов Е.А., Тимин Е.Н. и др. Влияние вторичных посредников на двигательную активность нейтрофилов. Вопросы медицинской химии 1994;6:7–10. [Galkin A.A., Tumanov E.A., Timin E.N. et al. Effect of secondary intermediaries on motility of neutrophils. Voprosy medizinskoy khimii = Medical Chemistry Issues 1994;6:7–10 (In Russ.)].
16. Галкин А.А., Туманов Е.А., Тимин Е.Н., Карелин А.А. Действие активаторов на подвижность нейтрофилов. Бюллетень экспериментальной биологии и медицины 1997;124(10):409–12. [Galkin A.A., Tumanov E.A., Timin E.N., Karelin A.A. Effect of activators on motility of neutrophils. Byulleten experimentalnoy biologii i mediziny = Bulletin of Experimental Biology and Medicine 1997;124(10):409–12 (In Russ.)].
17. Галкин А.А., Демидова В.С. Роль Са2+ в регуляции функций нейтрофилов. Успехи современной биологии 2007;127(1):58–72. [Galkin A.A., Demidova V.S. The role of Са2+ in regulation of functions of neutrophils. Uspekhi sovremennoy biologii = Progress of Modern Biology 2007;127(1):58–72 (In Russ.)].
18. Галкин А.А., Демидова В.С. Роль адгезии в активации нейтрофилов и цитотоксическом взаимодействии нейтрофилов с эндотелием. Успехи современной биологии 2011;131(1):62–78. [Galkin A.A., Demidova V.S. The role of adhesion in activation of neutrophils and cytotoxic interaction between neutrophils and the endothelium. Uspekhi sovremennoy biologii = Progress of Modern Biology 2011;131(1): 62–78 (In Russ.)].
Review
For citations:
Galkin А.А., Demidov V.S., Zakharova O.A. INHIBITION OF MOBILITY OF NEUTROPHILS OF SURGICAL PATIENTS WITH PURULENT WOUNDS AND WOUND INFECTION AS AN INDICATOR OF INTOXICATION OF THE ORGANISM. Wounds and wound infections. The prof. B.M. Kostyuchenok journal. 2014;1(2):38-43. (In Russ.) https://doi.org/10.25199/2408-9613-2014-1-2-38-43
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