Инновации в лечении ран различной этиологии. Лечение ран холодной плазмой: смена парадигмы (обзор литературы)
https://doi.org/10.25199/2408-9613-2026-13-2-16-22
Аннотация
Цель исследования – оценка результатов научных исследований по применению холодной плазмы в различных областях медицины.
Ранние исследования предоставили доказательства того, что плазма способствует заживлению ран благодаря полезному сочетанию активных веществ и модуляции воспаления в клетках и тканях. Холодную атмосферную плазму изучали и клинически применяли для лечения рака, пародонтита и других заболеваний, так как она оказывает цитотоксическое, бактерицидное, клеточно-пролиферативное и противовоспалительное действие на живые ткани путем образования реактивных частиц. Таким образом, холодная плазма является универсальным средством лечения заболеваний, связанных с хроническим воспалением и бактериальными инфекциями.
В данном обзоре представлены наиболее важные биомедицинские составляющие применения холодной атмосферной или низкотемпературной плазмы в современной медицине, демонстрирующие ее эффективность в заживлении хронических и острых ран.
Об авторах
И. Ю. КарповаРоссия
Карпова Ирина Юрьевна – доктор медицинских наук, доцент, заведующий кафедрой детской хирургии
603005, Нижний Новгород, пл. Минина и Пожарского, д.10/1
С. Л. Малиновская
Россия
Малиновская Светлана Львовна – доктор биологических наук, доцент, профессор кафедры медицинской биофизики
603005, Нижний Новгород, пл. Минина и Пожарского, д.10/1
Д. И. Иудин
Россия
Иудин Дмитрий Игоревич – доктор биологических наук, доктор физико-математических наук, профессор, заведующий кафедрой медицинской биофизики
603005, Нижний Новгород, пл. Минина и Пожарского, д.10/1
О. В. Другова
Россия
Другова Ольга Валентиновна – кандидат биологических наук., доцент кафедры медицинской биофизики
603005, Нижний Новгород, пл. Минина и Пожарского, д.10/1
Г. С. Розин
Россия
Розин Герман Сергеевич – студент 6-го курса педиатрического факультета
603005, Нижний Новгород, пл. Минина и Пожарского, д.10/1
Список литературы
1. Abdo A, Kopecki Z. Comparing Redox and Intracellular Signalling Responses to Cold Plasma in Wound Healing and Cancer. Curr Issues Mol Biol. 2024;46(5): 4885-4923. doi: https://doi.org/10.3390/cimb46050294
2. Nanbing Y, Yanzhi Y, Cuixia T, Xingwu R. Mechanism of cold atmospheric plasma in treatment of chronic skin ulcer. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2024;38(10):1283-1288. doi: https://doi.org/10.7507/1002-1892.202404027
3. Schmidt A, Woedtke T, Vollmar B, et al. Nrf2 signaling and inflammation are key events in physical plasma-spurred wound healing. Theranostics. 2019;9(4):1066-1084. doi: https://doi.org/10.7150/thno.29754
4. Holt A, Helder O, Vos M, et al. Antiseptic barrier cap effective in reducing central lineassociated bloodstream infections: A systematic review and meta-analysis. Int J Nurs Stud. 2017;69:34-40. doi: https://doi.org/10.1016/j.ijnurstu.2017.01.007
5. Bernhardt T, Semmler M, Schäfer M, et al. Plasma Medicine: Applications of Cold Atmospheric Pressure Plasma in Dermatology. Oxid Med Cell Longev. 2019; 201:1-10. doi: https://doi.org/10.1155/2019/3873928
6. Olsson M, Järbrink K, Divakar U, et al. The humanistic and economic burden of chronic wounds: A systematic review. Wound Repair Regen. 2019;27:114-125. doi: https://doi.org/10.1111/wrr.12683
7. Yuan N, Yang Y, Tan C, Ran X. Mechanism of cold atmospheric plasma in treatment of chronic skin ulcer. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2024; 38(10):1283-1288. doi: https://doi.org/10.7150/ijbs.71983
8. Ilut P, Păpara C, Danescu S, et al. Antibiotic susceptibility and resistance of bacterial Pathogens in chronic leg ulcers: a retrospective cohort study. Farmacia. 2023;71(1):38-43. doi: https://doi.org/10.1111/wrr.12683
9. Khalaf A, Abdalla A, Ren K, Liu X. Cold atmospheric plasma (CAP): a revolutionary approach in dermatology and skincare. Eur J Med Res. 2024;29(1):487. doi: https://doi.org/10.1186/s40001-024-02088-9
10. Gisele A, Philippe-Henri S, Lionel T, et al. Evolution of the Chronic Venous Leg Ulcer Microenvironment and Its Impact on Medical Devices and Wound Care Therapies. J Clin Med. 2023;12(17):5605. doi: https://doi.org/10.3390/jcm12175605
11. Olsson M, Järbrink K, Divakar U, et al. The humanistic and economic burden of chronic wounds: A systematic review. Wound Repair Regen. 2019;27:114-125. doi: https://doi.org/10.1111/wrr.12683
12. Martinengo L, Olsson M, Bajpai R, et al. Prevalence of chronic wounds in the general population: Systematic review and meta-analysis of observational studies. Ann Epidemiol. 2019;29:8-15. doi: https://doi.org/10.1016/j.annepidem.2018.10.005
13. Boekema B, Stoop M, Vlig M, et al. Antibacterial and safety tests of a flexible cold atmospheric plasma device for the stimulation of wound healing. Appl Microbiol Biotechnol. 2021;105(5):2057-2070. doi: https://doi.org/10.1007/s00253-021-11166-5
14. Braný D, Dvorská D, Strnádel J, et al. Effect of Cold Atmospheric Plasma on Epigenetic Changes, DNA Damage, and Possibilities for Its Use in Synergistic Cancer Therapy. Int J Mol Sci. 2021;22(22):12252. doi: https://doi.org/10.3390/ijms222212252
15. Braný D, Dvorská D, Strnádel J, et al. Effect of Cold Atmospheric Plasma on Epigenetic Changes, DNA Damage, and Possibilities for Its Use in Synergistic Cancer Therapy. Int J Mol Sci. 2021; 22(22):12252. doi: https://doi.org/10.3390/ijms222212252
16. Abdo A, Kopecki Z. Comparing Redox and Intracellular Signalling Responses to Cold Plasma in Wound Healing and Cancer. Curr Issues Mol Biol. 2024;46(5): 4885-4923. doi: https://doi.org/10.3390/cimb46050294
17. Gisele A, Philippe-Henri S, Lionel T, et al. Evolution of the Chronic Venous Leg Ulcer Microenvironment and Its Impact on Medical Devices and Wound Care Therapies. J Clin Med. 2023;12(17):5605. doi: https://doi.org/10.3390/jcm12175605
18. Ingleby A. The evolution of leg ulcer guidelines and recommendations. Br J Community Nurs.2023;28(12):22-30. doi: https://doi.org/10.12968/bjcn.2023
19. Rani R, Silina E, Stupin V, et al. Multifunctional and Smart Wound Dressings - A Review on Recent Research Advancements in Skin Regenerative Medicine. Pharmaceutics. 2022;14:1574. doi: https://doi.org/10.3390/pharmaceutics14081574
20. Rached N, Kley S, Storck M, et al. Cold Plasma Therapy in Chronic Wounds-A Multicenter, Randomized Controlled Clinical Trial (Plasma on Chronic Wounds for Epidermal Regeneration Study): Preliminary Results. J Clin Med. 2023;12(15): 5121. doi: https://doi.org/10.3390/jcm12155121
21. Ingleby A. The evolution of leg ulcer guidelines and recommendations. Br J Community Nurs. 2023;28(12):22-30. doi: https://doi.org/10.12968/bjcn.2023
22. Azzariti A, Iacobazzi R, Fonte R, et al. Plasma-Activated Medium Triggers Cell Death and the Presentation of Immune Activating Danger Signals in Melanoma and Pancreatic Cancer Cells. Sci Rep. 2019;9:1-13. doi: https://doi.org/10.1038/s41598-019-40637-z
23. Bruno M, Gagliardi A, Mancuso A, et al. Oleic acid-based vesicular nanocarriers for topical delivery of the natural drug thymoquinone: Improvement of anti-inflammatory activity. J Control Release. 2022;352:74-86. doi: https://doi.org/10.1016/j.jconrel.2022.10.011
24. Dubey S, Parab S, Amit AA, et al. Cold at mospheric plasma therapy in wound healing. Process Biochemistry. 2022;112:112-123. doi: https://doi.org/10.1016/j.procbio.2021.11.017
25. Murali R, Evangelina R, Samuel J, et al. Cold atmospheric plasma (CAP) in wound healing: harnessing a dual-edged sword. Redox Experimental Medicine. 2024. doi: https://doi.org/10.1530/REM-23-0026
26. Rached N, Kley S, Storck M, et al. Cold Plasma Therapy in Chronic Wounds - A Multicenter, Randomized Controlled Clinical Trial (Plasma on Chronic Wounds for Epidermal Regeneration Study): Preliminary Results. J Clin Med. 2023;12(15): 5121. doi: https://doi.org/10.3390/jcm12155121
27. Landscheidt K, Engelhardt C, Hernekamp J-H, Goertz O. Use of Cold Plasma in Wound Healing: A Case Report. Adv Skin Wound Care. 2022;35(12):1-3. doi: https://doi.org/10.1097/01.ASW.0000891084.22486.a7
28. Emmert S, Welzen A, Masur K, et al. Cold atmospheric pressure plasma for the treatment of acute and chronic wounds. Hautarzt. 2020;71(11):855-862. doi: https://doi.org/10.1007/s00105-020-04696-y
29. Hämmerle G, Ascher S, Gebhardt L. Positive effects of cold atmospheric plasma on pH in wounds: a pilot study. J Wound Care Actions. 2023;32(9):530-536. doi: https://doi.org/10.12968/jowc.2023.32.9.530
30. Sedik A, Salama M, Fathy K, Salama A. Cold plasma approach fortifies the topical application of thymoquinone intended for wound healing via up-regulating the levels of TGF-ß, VEGF, and α-SMA in rats. Int Immunopharmacol. 2023;122: 110634. doi: https://doi.org/10.1016/j.intimp.2023.110634
31. Marches A, Clement E, Albérola G, et al. Cold Atmospheric Plasma Jet Treatment Improves Human Keratinocyte Migration and Wound Closure Capacity without Causing Cellular Oxidative Stress. Int J Mol Sci. 2022;23(18):10650. doi: https://doi.org/10.3390/ijms231810650
32. Jensen J-O, Schulz L, Schleusser S, et al. The repetitive application of cold atmospheric plasma (CAP) improves microcirculation parameters in chronic wounds. Microvasc Res. 2021;138:104220. doi: https://doi.org/10.1016/j.mvr.2021.104220
33. Schleusser S, Schulz L, Song J, et al. A single application of cold atmospheric plasma (CAP) improves blood flow parameters in chronic wounds. Microcirculation. 2022;29(3):e12754. doi: https://doi.org/10.1111/micc.12754
34. Stratmann B, Costea T-C, Nolte C, et al. Effect of Cold Atmospheric Plasma Therapy vs Standard Therapy Placebo on Wound Healing in Patients With Diabetic Foot Ulcers: A Randomized Clinical Trial. JAMA Netw Open. 2020;3(7):e2010411. doi: https://doi.org/10.1001/jamanetworkopen.2020.10411
35. Cwajda-Białasik J, Mościcka P, Szewczyk M. Antiseptics and antimicrobials for the treatment and management of chronic wounds: a systematic review of clinical trials. Postepy Dermatol Alergol. 2022;39(1):141-151. doi: https://doi.org/10.5114/ada.2022.113807
36. Lee Y, Ricky S, Lim T, et al. An Atmospheric Plasma Jet Induces Expression of Wound Healing Genes in Progressive Burn Wounds in a Comb Burn Rat Model: A Pilot Study. J Burn Care Res. 2023;44(3):685-692. doi: https://doi.org/10.1093/jbcr/irab005
37. Duchesne C, Banzet S, Lataillade J, et al. Cold Atmospheric Plasma Modulates Endothelial Nitric Oxide Synthase Signalling and Enhances Burn Wound Neovascularisation. J Pathol. 2019;249:368-380. doi: https://doi.org/10.1002/path.5323
38. Matsumura K, Hayashi H, Uemura N, et al. Thrombospondin-1 overexpression stimulates loss of Smad4 and accelerates malignant behavior via TGF-beta signal activation in pancreatic ductal adenocarcinoma. Transl Oncol. 2022;26:101533. doi: https://doi.org/10.1016/j.tranon.2022.101533
39. Madamanchi A, Ingle M, Hinck A, Umulis D. Computational modeling of TGF-β2: TβRI: TβRII receptor complex assembly as mediated by the TGF-β coreceptor betaglycan. J Biophys. 2023;122:1342-1354. doi: https://doi.org/10.1016/j.bpj.2023.02.030
40. Liu M, Iosef C, Rao S, et al. Transforming Growth Factor-induced Protein Promotes NF-κB-mediated Angiogenesis during Postnatal Lung Development. Am J Respir Cell Mol Biol. 2021;64:318-330. doi: https://doi.org/10.1165/rcmb.2020-0153OC
41. Moaness M, Kamel A, Salama A, et al. Fast skin healing chitosan/PEO hydrogels: In vitro and in vivo studies. Int J Biol Macromol. 2024;265(Part 2):130950. doi: https://doi.org/10.1016/j.ijbiomac.2024.130950
42. Sharma S, Rai V, Narang R, Markandeywar T. Collagen-based formulations for wound healing: A literature review. Life Sciences. 2022;290:120096. doi: https://doi.org/10.1016/j.lfs.2021.120096
43. Elsayyad N, Salama A, Noshi S. Concurrent tissue engineering and infection prophylaxis utilising stable dual action amoxicillin loaded scaffolds. J Drug Del Sci Technol. 2020;58:101788. doi: https://doi.org/10.1016/j.jddst.2020.101788
44. Sigrid K, Stephanie A. Cold Plasma Treatment for Acute Wounds. Textbook of Good Clinical Practice in Cold Plasma Therapy. 2022;161–174. doi: https://doi.org/10.1007/978-3-030-87857-3_8
45. Li M, Gao J, Wang L, et al. Basic research and clinical exploration of cold atmospheric plasma for skin wounds. Bioeng Transl Med. 2023;8(5):e10550. doi: https://doi.org/10.1002/btm2.10550
46. Winter S, Meyer-Lindenberg A, Wolf G, et al. In Vitro Evaluation of the Decontamination Effect of Cold Atmospheric Argon Plasma on Selected Bacteria Frequently Encountered in Small Animal Bite Injuries. J Microbiol Methods. 2020; 169:105728. doi: https://doi.org/10.1016/j.mimet.2019.105728
47. Nishijima A, Fujimoto T, Hirata T, Nishijima J. Effects of Cold Atmospheric Pressure Plasma on Accelerating Acute Wound Healing: A Comparative Study among 4 Different Treatment Groups. Modern Plastic Surgery. 2019;9:18-31. doi: https://doi.org/10.4236/mps.2019.91004
48. Khabipov А, Schreiber А, Kersting S, et al. Cold Atmospheric Plasma Is a Promising Alternative Treatment Option in Case of Split Skin Graft Failure. Case Rep Surg. Open Access. 2024;1013445. doi: https://doi.org/10.1155/2024/1013445
49. Lee H, Kang S, Kim H, et al. Liquid plasma as a treatment for cutaneous wound healing through regulation of redox metabolism. Cell Death & Disease. 2023; 14(2):119. doi: https://doi.org/10.1038/s41419-023-05610-9.100
50. Lee H, Lee H, Kim G, et al. Plasma cupping induces VEGF expression in skin cells through nitric oxide-mediated activation of hypoxia inducible factor 1. Sci Reports. 2019;9(1):3821. doi: https://doi.org/10.1038/s41598-019-40086-8
51. Matzkeit N, Schulz L, Schleusser S, et al. Cold atmospheric plasma improves cutaneous microcirculation in standardized acute wounds: results of a controlled, prospective cohort study. Microvascular Research. 2021;138:104211. doi: https://doi.org/10.1016/j.mvr.2021.104211
Рецензия
Для цитирования:
Карпова И.Ю., Малиновская С.Л., Иудин Д.И., Другова О.В., Розин Г.С. Инновации в лечении ран различной этиологии. Лечение ран холодной плазмой: смена парадигмы (обзор литературы). Раны и раневые инфекции. Журнал имени проф. Б.М. Костючёнка. 2026;13(2):16-22. https://doi.org/10.25199/2408-9613-2026-13-2-16-22
For citation:
Karpova I.Yu., Malinovskaya S.L., Iydin D.I., Drugova O.V., Rozin G.S. Innovations in the treatment of various etiologies wounds. Cold plasma wound treatment: a paradigm shift (literature review). Wounds and wound infections. The prof. B.M. Kostyuchenok journal. 2026;13(2):16-22. (In Russ.) https://doi.org/10.25199/2408-9613-2026-13-2-16-22
JATS XML
























