Pharmacological effects of neostigmine during medicamental test with low frequency direct muscle stimulation by stimulative impedance myography method for thymectomy results control

Objective: a comparative analysis of the pharmacological effects of proserin during a drug test with low-frequency direct muscle stimulation by the method of stimulated impedance myography while controlling the effect of thimectomy.

Materials and methods. Patients suffered myasthenia gravis, which had thymoma, were investigated before and after thymectomy by means stimulative impedance myography method. Neostigmine test and low frequency 1.0 Hz direct needle muscle stimulation during 3 minutes were used. Amplitude of direct and transsynaptical evoked contractive reactions were measured in parallel researches.

Results. Results of researches of control group showed normal amplitude dispersion; meanwhile patients after neostigmine injection had right-side asymmetric dispersion and intermittent contractility. The amplitude transsynaptical contraction before thymectomy rose up on 14.0 %, meanwhile after operation – 29.0 %.

Conclusion. Probably neostigmine fell down threshold level of motor nerve terminals and made proposal to their excitationи by big impulses surrounded muscle fibers and impulses of stimulator.

1. Babinkov V. I., Losev N. I., Ippolitov I. Kh. Measurement of sensitivity to anticholinesterase drugs in patients after thimectomy = Babinkov V. I., Losev N. I., Ippolitov I. KH. Izmereniye chuvstvitel'nosti k antikholinesteraznym preparatam u bol'nykh posle timektomii. V kn.: Narusheniye mekhanizmov regulyatsii i ikh korrektsiya. Tezisy dokladov IV s"yezda patofiziologov 3–6 oktyabrya 1989, Kishinev. T. 1. M., 1989. S. 111. (In Russ.)]

2. Babinkov V. I. A method for determining the effectiveness of thimectomy in patients with myasthenia gravis = Babinkov V. I. Sposob opredeleniya effektivnosti timektomii u bol'nykh miasteniyey. Avtorskoye svidetel'stvo № 1678317. Byulleten' otkrytiy i izobreteniy. Publikatsiya 23.09.1991. № 35. (In Russ.)]

3. Babinkov V I. Losev N. I. Ipollitov I. Ch., et all. Analysis of amplitude distribution of the evoked contractive reactions of myasthenic muscle tissue during neostigmine test. Constituent Congress International society for pathophysiology. Moscow. May, 28 – June 1. 1991. Abstrats. p. 70.

4. Babinkov V. I. An experimental study of direct muscle excitability after synaptic blockade by stimulation impedance myography using volt-ohmic characteristics = Babinkov V. I. Eksperimental'noye issledovaniye pryamoy myshechnoy vozbudimosti posle sinapticheskoy blokady metodom stimulyatsionnoy impedansnoy miografii, ispol'zuya vol't-omicheskiye kharakteristiki. Rany i ranevyye infektsii. 2017; 4 (4): 12–17. (In Russ.)

5. The physiology of synapses. By John Carew Eccles. Springer-Verlag. Berlin: Gottinger Heldelberg, 1964. (In Russ.)]

6. Bernard Katz. Nerve, Muskle and synaps. New York, 1966 (In Russ.)]

7. Afzalov R. A. The mechanism of re-activity in the neuromuscular synapse of coldblooded = Afzalov R. A. Mekhanizm povtornoy aktivnosti v nervno-myshechnom sinapse kholodnokrovnykh. Avtoref. diss. ... kand. biol. nauk. Kazan', 2000. (In Russ.)

8. Babinkov V. I., Yakovenko V. N. Theoretical basis of the method of measuring direct muscle excitability (experimental study) = Babinkov V. I., Yakovenko V. N. Teoreticheskaya osnova sposoba izmereniya pryamoy myshechnoy vozbudimosti (eksperimental'noye issledovaniye). Rany i ranevyye infektsii. 2017; 4 (2): 12–17. (In Russ.)]

9. Sanadze A. G. Mechanisms of formation and clinical significance of electrophysiological phenomena associated with pathology of neuromuscular transmission = Sanadze A. G. Mekhanizmy formirovaniya i klinicheskaya znachimost' elektrofiziologicheskikh fenomenov, svyazannykh s patologiyey nervno-myshechnoy peredachi. V kn.: Elektromiograficheskiye metody izucheniya funktsional'nogo sostoyaniya dvigatel'nykh yedinits skeletnykh myshts v norme i patologii. M., 1988. S. 58–78. (In Russ.)]

10. Kaminsky L. S. Processing of clinical and laboratory data. The use of statistics in the scientific and practical work of a doctor = Kaminskiy L. S. Obrabotka klinicheskikh i laboratornykh dannykh. Primeneniye statistiki v nauchnoy i prakticheskoy rabote vracha. Medgiz. Leningradskoye otdeleniye, 1959. (In Russ.)

11. Gurfinkel V. S., Levik Yu. S. Skeletal muscle: structure and function = Kaminskiy L. S. Obrabotka klinicheskikh i laboratornykh dannykh. Primeneniye statistiki v nauchnoy i prak

12.

13. Sanadze A. G. Myasthenia gravis and myasthenic syndromes = Sanadze A. G. Miasteniya i miastenicheskiye sindromy. M.: GEOTAR-media, 2019. (In Russ.)