ririРаны и раневые инфекции. Журнал имени проф. Б.М. КостючёнкаWounds and wound infections. The prof. B.M. Kostyuchenok journal2408-96132500-0594Regional non-governmental organization “Surgical society - Wounds and wound infections”10.17650/2408-9613-2015-2-4-8-14riri-62Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL REPORTSСРАВНИТЕЛЬНАЯ ХАРАКТЕРИСТИКА ЗАЖИВЛЕНИЯ ХИРУРГИЧЕСКИХ РАН СЛИЗИСТОЙ ОБОЛОЧКИ ПОЛОСТИ РТА И КОЖИ У ДОМАШНИХ СВИНЕЙ. СВЕТООПТИЧЕСКОЕ И ЭЛЕКТРОННО-МИКРОСКОПИЧЕСКОЕ ИССЛЕДОВАНИЕCOMPARATIVE DESCRIPTION OF HEALING OF SURGICAL WOUNDS IN THE ORAL MUCOSA AND SKIN OF PIGS. LIGHT OPTICAL AND ELECTRON MICROSCOPIC EXAMINATIONРагимовЧ. Р.RagimovCh. R.

Кафедра хирургии полости рта и челюстно-лицевой области

Oral and Maxillofacial Surgery Department

ГасымовЭ. К.GasymovE. K.

Кафедра гистологии, эмбриологии и цитологии.

Контакты: Эльдар Кочари оглы Гасымов.Азербайджанская Республика, 1022, Баку, ул. Миргасымова, 1

Department of Histology, Embryology, and Cytology. 

Address: 1 Mirgasymova St., Baku, 1022, Republic of Azerbaijan

geldar1949@gmail.comКулиевТ. Р.KulievT. R.

Кафедра хирургии полости рта и челюстно-лицевой области

Oral and Maxillofacial Surgery Department

РзаевФ. Г.RzaevF. G.

Отдел электронной микроскопии

Department of Electron Microscopy

Азербайджанский медицинский университет, БакуАзербайджанAzerbaijan Medical University, BakuAzerbaijan20153006201624814Copyright © Рагимов Ч.Р., Гасымов Э.К., Кулиев Т.Р., Рзаев Ф.Г., 20162016Рагимов Ч.Р., Гасымов Э.К., Кулиев Т.Р., Рзаев Ф.Г.Ragimov C.R., Gasymov E.K., Kuliev T.R., Rzaev F.G.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.riri.su/jour/article/view/62

Цель исследования – изучить особенности структурных элементов твердого неба и кожи при заживлении резано-ушитых ран на стадии гемостаза и воспаления у домашних свиней.

Материалы и методы

Материалы и методы. Для эксперимента были взяты 9 поросят с массой тела 35–40 кг. На коже спины и в твердом небе были сделаны прямолинейные разрезы (модели хирургических ран) длиной 2,5–3,0 см. Для проведения морфологических исследований в каждой серии экспериментов (контроль, через 6 ч и на 3-е и 7-е сутки после операции) было взято по 3 биоптата как из кожи, так и из слизистой оболочки твердого неба. Материал для электронной микроскопии фиксировали иммерсией in situ и далее об- рабатывали по общепринятой методике. Полу- и ультратонкие срезы изучали соответственно под световым микроскопом Latimet (Leica) и электронным микроскопом JEM-1400 (JEOL) при ускоряющем напряжении 80–120 кВ.

Результаты

Результаты. Быстрое угасание воспалительных процессов резано-ушитых ран кожи на ранних этапах заживления приводит к задержке очистки поврежденных структур и фибриноидов с раневой поверхности по сравнению с таковыми на слизистой обо- лочке твердого неба. Поэтому формирование созревшей грануляционной ткани на коже начинается не на 3-и, а на 7-е сутки.

Выводы

Выводы. Различия в фазах гемостаза и воспаления отражаются на последующих фазах репаративной регенерации (пролиферации и формирования рубца), что, возможно, и приводит к различию в развитии рубцовой ткани послеоперационных ран. 

Objective

Objective: to study the specific features of the structural elements of the hard palate and skin in the healing of incised and sutured wounds at the stage of hemostasis and inflammation in pigs.

Materials and methods

Materials and methods. Nine piglets weighing 35–40 kg were taken for an experiment. 2.5–3.0-cm rectilinear incisions (surgical wound models) were made in the back skin and hard palate. Three skin biopsy specimens and 3 hard palate mucosal biopsy specimens were taken for morphological examinations in each experimental series (control, 6 hours and 3 and 7 days postsurgery). The electron microscopic material was fixed by an in situ immersion method and then processed by the conventional procedure. Semi- and ultrafine sections were examined under a Latimet light microscope (Leica) and a JEM-1400 electron microscope (JEOL), respectively, at an accelerating voltage of 80–120 kW.

Results

Results. A rapid decrement of inflammatory processes in the incised and sutured wounds in the early stages of healing results in delayed cleansing of damaged structures and fibrinoids from the wound surface compared to those in the heard palate mucosa. So formation of mature skin granulation tissue begins on day 7 rather on day 3.

Conclusion

Conclusion. The differences in the phases of hemostasis and inflammation affect the further phases of reparative regeneration (proliferation and scar formation), which may lead to a difference in the development of postoperative wound scar tissue. 

модель хирургической раныкожаслизистая оболочка твердого небагемостазвоспалениетрансмиссионный электронный микроскопрепаративная регенерацияфибринкератиноцитытонофибриллыфибриноиднейтрофильная и макро- фагальная инфильтрацияфагоцитозапоптотические тельцаонкотический некрозгрануляционная тканьsurgical wound modelskinhard palate mucosahemostasisinflammationtransmission electron microscopereparative regenerationfibrinkeratinocytestonoflbrilsfibrinoidneutrophil and macrophage infiltrationphagocytosisapoptotic bodiesoncotic necrosisgranulation tissueReferencesOdland G., Ross R. Human wound repair. I. Epidermal regeneration. J Cell Biol 1968;39(1):135–51.Odland G., Ross R. Human wound repair. I. Epidermal regeneration. J Cell Biol 1968;39(1):135–51.Alster T.S., Tanzi E. Hypertrophic scars and keloids. Clin Dermatol 2003;4(4):235–43.Alster T.S., Tanzi E. Hypertrophic scars and keloids. Clin Dermatol 2003;4(4):235–43.Pastar I., Stojadinovic O., Yin N.C. et al. 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The authors declare that there are no conflicts of interest present.